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Robustness of HPTLC method at the concentration of 200 ng/band (n = 6).
| Conc. (ng/Bnd) | Mobile Phase Composition (EA:Methanol:Ammonia) | Recovery (%) ± SD | RSD (%) | Rf | ||
|---|---|---|---|---|---|---|
| Original | Used | Level | ||||
| 200 | 5:4:2 | 4.9:4.1:2 | +0.1 | 95.38 ± 2.69 | 2.82 | 0.41 |
| 5:4:2 | 0.0 | 104.10 ± 3.11 | 2.98 | 0.42 | ||
| 5.1:3.9:2 | −0.1 | 103.61 ± 3.74 | 3.60 | 0.43 | ||
