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. 2020 Mar 5;7(8):1900997. doi: 10.1002/advs.201900997

Figure 2.

Figure 2

TA‐cloning analysis of s4U‐to‐C conversion in untreated CCND1 mRNA. A) Sanger sequencing results of the specific region (chr 11, 69 652 513–69 652 529). B) The mutation sites of T‐to‐C which indicated the incorporation of s4U. C) The average T‐to‐C mutation rate in the selected region (s4U(−)C2H3CN(−), n = 16), CCND1 mRNA treated acrylonitrile (s4U(−)C2H3CN(+), n = 24), s4U‐tagged CCND1 mRNA (s4U(+)C2H3CN(−), n = 25), and acrylonitrile‐treated s4U‐tagged CCND1 mRNA (s4U(+)C2H3CN(+), n = 10). “n” stands for the number of TA‐cloning samples used for each statistical analysis.