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. 2020 Feb 25;7(8):1903332. doi: 10.1002/advs.201903332

Figure 3.

Figure 3

Chemophysical characterization of the prodrug nanovectors. a) UV–vis spectra of PPa, NSP, NCP, HCNSP, and HCNCP at the identical PPa concentration. b) Fluorescence spectra properties of PPa, NSP, NCP, HCNSP, and HCNCP at the identical PPa concentration. The influence of GSH and SDS incubation on the fluorescence features of c) HCNSP, and d) HCNCP, respectively (the inset showed the fluorescence imaging of two prodrug nanovectors). e) NLG919 release profile of HCNSP and HCNCP with or without incubation with 10 × 10−3 m GSH. f) Laser‐induced ROS generation of the HCNSP and HCNCP as determined by using ABDA as an indicator, the inset showed the UV–vis spectra characterization. g) CLSM images of intracellular distribution of CNSP, HCNSP, and HCNSP (scale bar = 25 µm). h) Flow cytometric detection of cellular uptake of CNSP, HCNSP, the cells of the HCNSP group were pretreated with free HA. i) Flow cytometric study of intracellular uptake of HCNSP in CT26 cells as a function of incubation time.