Table 1.
List of plasmids and bacterial strains used in this study. DSMZ: Leibiz Institute DSMZ-German Collection of Microorganisms and Cell Cultures. KmR: Resistance to kanamycin, RIDL: Research Institute Dupuy de Lôme, UBS: Université de Bretagne Sud.
| Strain or Plasmid | Characteristics | Origin |
|---|---|---|
| Halomonas strain | ||
|
Halomonas sp. SF2003 ID CNCM-I-4786 |
Wild type PHA-producing strain | Sea of Iroise (France), RIDL Collection, UBS |
| Cupriavidus necator strains | ||
| H16 (DSM 428) | Wild type PHA-producing strain | DSMZ Collection |
| PHB¯4 (DSM 541) | Mutant non-PHA-producing strain | DSMZ Collection |
| Escherichia coli strains | ||
| E. cloni® 10G | Competent cells | Lucigen |
| S17-1 | Strain for conjugative transfer of plasmid to C. necator PHB¯4 | Simon et al. 1983 [14] |
| Plasmids | ||
| pBBR1-ProCn | pBBR1MCS-2 derivatives with phaC1 promoter from C. necator. KmR. |
Foong et al. 2014 [15] |
| pBBR1-ProCn-phaC1 | pBBR1MCS-2 derivatives with phaC1 promoter from C. necator and phaC1 of Halomonas sp. SF2003. KmR. |
This study |
| pBBR1-ProCn-phaC2 | pBBR1MCS-2 derivatives with phaC1 promoter from C. necator and phaC2 of Halomonas sp. SF2003. KmR. |
This study |
| Transformants | ||
| PHB¯4/pBBR1-ProCn-phaC1 | Transformant strain with pBBR1MCS-2 plasmid expressing phaC1 of Halomonas sp. SF2003. KmR. |
This study |
| PHB¯4/pBBR1-ProCn-phaC2 | Transformant strain with pBBR1MCS-2 plasmid expressing phaC2 of Halomonas sp. SF2003. KmR. |
This study |