Figure 1.

Knockouts of helicase-like transcription factor (HLTF) and SNF2, histone-linker, PHD and RING finger domain-containing helicase (SHPRH) reduces sensitivity towards several DNA damaging agents. (A) Viability over 3–4 days was measured in all cell lines after exposure to mitomycin-C (MMC) (0.05 μM), cisplatin (1 μM), methyl methanesulfonate (MMS) (50 μM) or UV (50 mJ/cm²). Change in viability in treated versus untreated ko cell normalized to similarly treated parental cells are shown, i.e., value of 1 represents the same response as in parental cells (3 independent experiments ± SD are shown). Representative parental cell response to the treatments are shown in Figure A2. (B) Normal growth of untreated cell lines. Two-sided student’s t-test, * p < 0.05, ** p < 0.01.