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. 2020 Mar 17;10(3):463. doi: 10.3390/biom10030463

Figure 2.

Figure 2

HLTF reduces error-prone DNA damage tolerance (DDT) after UVB-irradiation. (A) Mutation frequencies after overexpression of UVB damaged supF reporter plasmid pSP189 relative to control (Hap1 parent cell line) with a number of colonies counted for HLTF ko (n = 11,265), SHPRH ko (n = 25,059), HLTF/SHPRH dko (n = 13,325), Parent (n = 25,725), two-sided student’s t-test, * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Quantification of types of mutations from sequencing mutant colonies from (A). Mutations with a prevalence ≥ 2% are depicted. Mutations found at T or C bases in supF are counted as mutations originated on the (sequenced) coding strand, mutations at A or G bases are considered to be C and T mutations originating from the transcribed strand. HLTF ko (n = 141), SHPRH ko (n = 112), HLTF/SHPRH dko (n = 127), Parent (n = 282). (C) Mutations at positions 155, 156, 164, 172 in supF gene from SupF UVB (A) in % with a prevalence of ≥ 10% in isolated plasmids from at least one cell line. (D) Mutation spectra of the supF gene received from sequencing mutant colonies from (A). HLTF ko (n = 141), SHPRH ko (n = 112), HLTF/SHPRH dko (n = 127), Parent (n = 282).