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Effect of PDTC on APP and p65 induction by CuONPs. SH-SY5Y cells were plated at 2 × 106 cells/well (6 well plate) and exposed to (A) TNFα (50 ng/mL) or (B) CuONPs (10 μM) in the presence or absence of PDTC (50 nM) for 6 h. Cells were harvested and lysates (nuclear and cytoplasmic) blotted for p65, APP, and β-actin by Western blotting.
