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Effect of p65 knockdown on amyloid precursor protein (APP) induction by CuONPs and TNFα. Effects of p65 siRNA (15 nM) on APP induction by CuONPs and TNFα were assessed. SH-SY5Y cells were plated at 2 × 10 6 /well and transfected with either control siRNA or p65 siRNA. After 24 h, transfected cells were exposed to either CuONPs (10 μg/mL) or TNF-α (50 ng/mL) for 6 h. Cells were lysed and lysates blotted for p65 (A) or APP (B), and β-actin as a control.
