Table 1.
Thermodynamic parameters of myoglobin in the absence and presence of different concentrations of ficoll 70 at different pH values.
| pH | [Ficoll 70] mg/mL |
GdmCl-Induced Denaturation | Urea-Induced Denaturation | ||
|---|---|---|---|---|---|
| ΔGD°(kcal mol−1) | Cm[M] | ΔGD°(kcal mol−1) | Cm[M] | ||
| 6 | 0 | 10.13 ± 0.11 (10.15 ± 0.12) * |
1.26 ± 0.04 (1.25 ± 0.05) * |
10.25 ± 0.18 (10.23±0.15) * |
3.99 ± 0.05 (4.00 ± 0.04) * |
| 100 | 9.70 ± 0.18 | 1.15 ± 0.06 | 9.75 ± 0.15 | 3.60 ± 0.03 | |
| 200 | 9.22 ± 0.12 | 1.00 ± 0.05 | 9.31 ± 0.11 | 3.30 ± 0.04 | |
| 300 | 8.78 ± 0.13 | 0.95 ± 0.05 | 8.72 ± 0.10 | 3.07 ± 0.06 | |
| 7 | 0 | 11.10 ± 0.14 (11.13 ± 0.16) * |
1.53 ± 0.03 (1.56 ± 0.02) * |
10.95 ± 0.15 (10.98 ± 0.13) * |
5.10 ± 0.04 (5.09 ± 0.05) * |
| 100 | 10.34 ± 0.15 | 1.4 ± 0.04 | 10.38 ± 0.16 | 4.63 ± 0.05 | |
| 200 | 9.87 ± 0.11 | 1.2 ± 0.05 | 9.87 ± 0.14 | 4.15 ± 0.06 | |
| 300 | 9.08 ± 0.14 | 1.1 ± 0.03 | 9.11 ± 0.13 | 3.58 ± 0.04 | |
| 8 | 0 | 10.23 ± 0.15 (10.21 ± 0.13) * |
1.82 ± 0.04 (1.81 ± 0.03) * |
10.25 ± 0.11 (10.23 ± 0.14) * |
5.83 ± 0.04 (5.85 ± 0.03) * |
| 100 | 9.76 ± 0.11 | 1.7 ± 0.03 | 9.71 ± 0.18 | 5.46 ± 0.05 | |
| 200 | 9.28 ± 0.13 | 1.65 ± 0.04 | 9.28 ± 0.13 | 5.04 ± 0.05 | |
| 300 | 8.81 ± 0.14 | 1.60 ± 0.05 | 8.81 ± 0.16 | 4.75 ± 0.06 | |
* Values given in parenthesis are from Δɛ409 measurements. ‘±’ sign with each parameter represents the mean error obtained from the triplicate measurements.