Table 2. The effect of the volume of seminal plasma (SP) infused into the vagina on endometrial epidermal growth factor (EGF) concentrations.
| SP (ml) | PBS (ml) | n | No. (%) of cows with normal EGF concentrations on day 3 after infusion | Endometrial EGF concentrations (ng/g tissue weight) | ||
|---|---|---|---|---|---|---|
| Before infusion | After infusion | |||||
| All cows | Normalized cows * | |||||
| 0.5 | 0 | 8 | 4 (50.0) | 1.83 ± 0.94 a | 4.84 ± 1.54 bB | 6.01 ± 0.76 A (4) | 
| 10 | 0 | 8 | 5 (62.5) | 1.83 ± 1.36 a | 5.93 ± 1.83 bB | 7.21 ± 0.61 B (5) | 
| 0.5 | 9.5 | 8 | 5 (62.5) | 1.84 ± 1.34 a | 5.91 ± 2.29 bB | 7.46 ± 0.76 B (5) | 
| 0 | 10 | 8 | 2 (25.0) | 1.95 ± 1.24 | 2.55 ± 1.92 A | 5.35, 5.86 (2) | 
Values are means ± SDs. * The numbers in the parentheses are the number of cows with normalized EGF concentrations after infusion. The phosphate-buffered saline (PBS) infusion (control) group was excluded from statistical analysis due to its small number. a, b Means with different letters within the same row differ (P ˂ 0.01). A, B Means with different letters within the same column differ (P ˂ 0.01).