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| Gene | Forward primer (5'→3') | Reverse primer (5'→3') | Product size (bp) | Accession No. |
|---|---|---|---|---|
| Fshb | CTGCTACACTAGGGATCTGG | TGACATTCAGTGGCTACTGG | 156 | NM_008045 |
| Lhb | CTAGCATGGTCCGAGTACTG | CCCATAGTCTCCTTTCCTGT | 136 | NM_008497 |
| Gnrh-r | CAGGATGATCTACCTAGCAG | GCAGATTAGCATGATGAGGA | 154 | NM_010323 |
| Gpr120 | TCGCTGTTCAGGAACGAATG | CACCAGAGGCTAGTTAGCTG | 130 | NM_181748 |
| L19 | CCAAGAAGATTGACCGCCATA | CAGCTTGTGGATGTGCTCCAT | 101 | NM_009078 |
