Fig. 3.

Knockdown of retinoblastoma binding protein 7 (RBBP7) expression repressed Kiss1 mRNA expression in the mHypo-A55 cells. Rbbp7 mRNA expression in mHypoA-55 cells treated with E2 following Rbbp7 siRNA transfection (A). NC, negative control siRNA; #1 and #2, two sets of siRNA against Rbbp7. The mRNA levels were measured by quantitative RT-PCR. Representative western blot bands and results of densitometric analysis of RBBP7 protein expression (B). The band intensities were estimated by densitometry using Image J. Kiss1 mRNA expression in mHypoA-55 cells treated with E2 following Rbbp7 siRNA transfection (C). Expression levels were normalized to Actb mRNA or β-actin protein. Data are the mean ± SEM (n = 3) and are expressed as % of controls treated with vehicle (Veh) and NC siRNA. * P < 0.05, two-way ANOVA with Bonferroni post-hoc test.