Skip to main content
. 2020 Jan 19;66(2):125–133. doi: 10.1262/jrd.2019-149

Fig. 4.

Fig. 4.

Rbbp7 and Kiss1 expression in mHypoA-55 cells and in the arcuate nucleus (ARC) and anteroventral periventricular nucleus (AVPV) regions of ovariectomized (OVX) rats treated with E2. Rbbp7 expression in mHypoA-55 cells treated with E2 for 2 h (A). Kiss1 expression levels in mHypoA-55 cells treated with E2 for 2 h (B) and 4 h (C), respectively. Expression levels were normalized to Actb mRNA level. Data are the mean ± SEM (n = 5–6) and are expressed as % of vehicle-treated controls. Relative Rbbp7 expression levels in the ARC (D) and AVPV (E) of OVX rats treated with diestrous levels (low) or proestrous levels (high) of E2. Relative Kiss1 expression levels in the ARC (F) and AVPV (G) of OVX rats treated with low or high E2. The mRNA levels were measured by quantitative RT-PCR. Data are the mean ± SEM (n = 5–6) and are expressed as the relative ratio of Rbbp7 or Kiss1 mRNA to Actb mRNA. * P < 0.05, one-way ANOVA with Bonferroni post-hoc test.