Table 4.

Summary of Experimental and Simulation Findings for Each Peptide

Peptide	Experiment	Simulation
Melittin	Transient pores at high peptide concentration (e.g., P:L ≥ 1:50) (18,20)	Very small and unstable pore with four peptides. Two peptides leave the pore.
MelP5	Active at lower peptide concentrations than melittin (e.g., P:L = 1:500) (20). Requires higher peptide concentrations than pHD15 at low pH and mac70 (22).	Small but stable pore
pHD15, pH 7	Peptide is random coil and does not bind the membrane (21,24).	Helices unfold and come to the surface. Water pore seems unstable.
pHD15, pH 5	Potent pore former at very low peptide concentrations (21)	A large pore forms when Ct is protonated as well as Glu, Asp, and His residues.
mac70, neutral and low pH	Potent pore former at low peptide concentrations (22)	Large pores at neutral pH and low pH where Glu residues and Ct are protonated
MelP5_Δ6	Designed to be active at low pH, but needs high concentrations of peptide and only makes small pores (23)	Small, defect-like pore at low pH