Table 1:
Specific primer pairs for amplification of the investigated and reference genes.
| Gene | Gene ID (NCBI-NLM database) | Primer | Tm,0 C | Product length (bp) | Exon junction |
|---|---|---|---|---|---|
| Foxp3 | NM_001108250.1 | F = TCTGGCCAAAAGACAGGTGG R = CTGTCCCAGGGTCCACAAAG | 60 60 |
40 | 2577/2578 |
| mTOR | NM_019906.1 | F = CGAGACTTGGAAGTCAGCCAC R = TCTGAGGCAGGCTGGATAACG | 60 61 |
61 | 214/215 |
| IL1β | NM_031512.2 | F = TCTTTGAAGAAGAGCCCGTCC R = GGTCGTCATCATCCCACGAG | 60 60 |
48 | 354/355 |
| IL17a | NM_001106897.1 | F = CTGGACTCTGAGCCGCAATG R = TGCCTCCCAGATCACAGAAG | 61 59 |
58 | 297/298 |
| GAP DH | F = GCCTGGAGAAACCTGCCAAG R = GCCTGCTTCACCACCTTCT | 61 60 |
52 | 825/826 |