Table 1. Peripheral Proteins Tested and CG Simulation Details^f.

protein	PDB id	bilayer composition	test type	force field	starting configuration	simulation length (ns)	no. of simulations	total time (ns)
BtPI-PLC	3EA1/2OR2	DMPC	MBTa	2.2P	MBOc	500	5	2500
			MDTb	2.2P	MBSd	500	5	2500
			MBT	2.3P	MBO	500	5	2500
			MBT	2.3P	ROe	1000	5	5000
PH domain (PLC-δ1)	1MAI	POPC/POPS/PIP2/PIP3 (73:20:5:2)	MBT	2.2P	RO	1000	1	1000
			MBT	2.3P	RO	1000	1	1000
PR3	1FUJ	POPC	MBT	2.2P	MBO	1000	1	1000
			MBT	2.3P	MBO	1000	1	1000
Equinatoxin	1IAZ	POPC	MBT	2.2P	MBO	500	1	500
			MBT	2.3P	MBO	500	1	500
PLA2	1POA	DMPC	MBT	2.2P	RO	1000	1	1000
			MBT	2.3P	RO	1000	1	1000
PC-specific SaPI-PLC	4I8Y	POPC	MBT	2.2P	RO	1000	1	1000
			MBT	2.3P	RO	1000	1	1000
WT SaPI-PLC	4F2T	POPC	MBT	2.3P	RO	500	2	1000
GLTP	1WBE	POPC	MBT	2.2P	RO	400 + 362	2	762
			MBT	2.3P	RO and MBO	500	2	1000

^aMBT: membrane binding test.

^bMDT: membrane detachment test.

^cMBO: membrane-binding orientation.

^dMBS: membrane-bound structure.

^eRO: randomized orientation.

^fTests with other interaction levels are reported in Table S1 and are not included here.