Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 22;17:123. doi: 10.1186/s12974-020-01795-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — ET-1-induced scratching is not affected by mMCP5 deficiency. aCpa3^-/- mice (n = 10) are deficient in both CPA3 and mMCP5, and had more frequent scratching bouts in 60 min than wild-type controls (WT, n = 10) when injected with ET-1 (20 pmol). bCpa3^-/- mice also spent more time scratching during the 60 min. c No difference was seen between genotypes in the mean length of scratching episodes. d When the frequency results from (a) were analyzed over time, it was seen that Cpa3^-/- mice scratched significantly more than controls overall (indicated by vertical significance bar), and post hoc analysis showed a significant difference in the interval between 20 and 30 min. e, f Visual comparison of the total frequency of scratching episodes and scratching frequency development over time, between Cpa3^-/- mice (data from (a)), Cpa3^Y356L,E378A mice (Cpa3^Y3, data from Fig. 2e) and Mcpt4^-/-Mcpt6^-/-Cpa3^-/- mice (data from Fig. 2a). The two CPA3-deficient mouse lines follow a similar scratching pattern and magnitude, while the Mcpt4^-/-Mcpt6^-/-Cpa3^-/- mice demonstrate more exaggerated behavior. Results are presented as mean ± SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, Student’s t test (a-c), 2-way ANOVA with Bonferroni multiple comparison (d)