FIG. 1.
CFTRact-K267 activation of CFTR chloride conductance at the ocular surface in mice studied by ocular surface PD measurements. (A) Schematic of the method showing perfusion of the ocular surface with different solutions and measurement of PD using a high-impedance voltmeter, with sensing electrode in electrical contact with the perfusion solution and grounding needle electrode placed under the skin. (B, C) (Left) Representative continuous recordings of ocular surface PD as a function of time in response to perfusion with solutions containing indicated compounds (see the Methods section for solution compositions). Concentrations of CFTRact-K267 were 10 μM (B) and 2.5 μM (C). (Right) Summary of changes in ocular surface PD (ΔPD) for successive solution changes. Each point is from a study performed on a different eye (mean ± SEM). CFTR, cystic fibrosis transmembrane conductance regulator; PD, potential difference; SEM, standard error of the mean.