FIG. 2.
Ocular pharmacokinetics of CFTRact-K267. (A) LC/MS assay of CFTRact-K267 amount in tear fluid at 0.5, 1, 2, 3, and 6 h after single-dose (2 nmol) administration as 2 μL eye drop. Representative chromatograms (m/z = 332) shown, with standard curve shown as inset. (B) Averaged recovered CFTRact-K267 (left ordinate) and deduced CFTRact-K267 concentration (right ordinate) computed assuming a 0.5 μL tear fluid volume per eye (mean ± SEM, 4 eyes per time point). LC/MS, liquid chromatography/mass spectroscopy.