Figure 5.

The TZF domain of MEX-5_CX10C is folded in the RNA-free state. (A) The ¹⁵N-¹H HSQC spectrum of MEX-5_CX10C TZF domain shows 78 crosspeaks, indicating that the domain is folded. (B) Shown is the Zn²⁺ titration of the TZF domain of MEX-5 and of the variant MEX-5_CX10C, followed by NMR spectroscopy. The average crosspeak intensities from the ¹⁵N-¹H HSQC spectra are shown as a function of zinc equivalents added to the protein, errors are estimated from the standard deviation. A linear regression is applied to the first part of each curve, and the resulting slope is shown in the plot. To see this figure in color, go online.