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. 2020 Mar 29;118(8):1861–1875. doi: 10.1016/j.bpj.2020.03.011

Figure 4.

Figure 4

Analysis of dimerization of RHBDL2 and GCPII in GPMVs derived from HeLa cells by FRET measurements and MC simulations. (A) A comparison of acceptor-concentration-dependent FRET efficiencies obtained experimentally (black squares) and from MC simulations (circles) is shown. The MC simulations were carried out for RHBDL2 and GCPII at increasing KD-values and for excluded radii ranging from 30 to 60 Å and from 20 to 70 Å for RHBDL2 and GCPII, respectively. Donor concentrations are not depicted for the sake of simplicity, but for every analyzed GPMV, they were used as input parameters for the MC simulations. (B) Heat maps of −log (MSD) visualizing the agreement between the measured and the simulated data for various values of KD and the excluded radii are shown. Table S2 lists all concentrations of donor and acceptors. pKD = −log(KD); MSD, mean-square deviation.