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. Author manuscript; available in PMC: 2020 Apr 22.
Published in final edited form as: Bioconjug Chem. 2018 Jun 12;29(7):2195–2207. doi: 10.1021/acs.bioconjchem.8b00104

Figure 4.

Figure 4.

Representative ESI-MS spectra of samples containing a 1:10 molar ratio of either TAR (A) or cTAR (B) and D-4h (red ●) compound in 150 mM ammonium acetate (see Experimental Section). Symbol ◼ corresponds to TAR RNA substrate and ◻ to cTAR DNA substrate. The stoichiometries of the observed complexes are indicated in each spectrum. Signals of lower intensity detected near free/bound species consist of typical sodium and ammonium adducts. (C) Histograms displaying the percentages of bound substrate observed in A and B spectra and from Figure S4 for D-4b and D-4h. The indicated percentages of bound substrate provide a measure of the relative affinities of d-analogues for either nucleic acid substrate.