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ESI-MS spectrum of samples obtained by adding ligand D-4h to the preformed TAR●NC complex (case i in Scheme 3). Lower intensity signals near free/bound species consist of typical sodium and ammonium adducts. Gray ▲ corresponds to NC protein; ◼ to the TAR RNA substrate; and red ● to the D-4h ligand. For the sake of clarity only the 6- and 7-charge states of the binary (RNA-ligand) and ternary (RNA-ligand-protein) complexes are labeled in the spectrum.
