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. Author manuscript; available in PMC: 2021 Mar 31.
Published in final edited form as: Biochemistry. 2020 Mar 17;59(12):1217–1220. doi: 10.1021/acs.biochem.0c00103

Figure 4.

Figure 4.

Bacterial growth inhibition by roxithromycin (pH 8.9) in cultures overexpressing Rob-wt protein or suppression of a Rob gene having a TAG codon at protein position 40 by a specific amino acid. Abbreviations: wt, wild type; no, no Rob protein sequence in the vector; Rob40–1, Rob40–2, Rob40–3, Rob40–4, Rob40–F, and Rob40–L, Rob40 with nucleobase amino acids 1, 2, 3, 4, Phe, and Leu at position 40 instead of Arg, respectively. Rob40–1 vs Rob-wt: p < 0.05. Rob40–2 vs Rob-wt: p > 0.05.