Figure 3.

Downregulation of piR-004800 induced autophagic death in MM cells. (A–C) RPMI8226 and U266 cells were transfected with antagomir-NC or antagomir-4800, and harvested 48 h later, then Western blot analysis was explored. (D) RPMI8226 cells were transfected with antagomir-NC or antagomir-4800, 48 h later the cells were treated with 3MA for 24 h, then cell inhibition rate was assessed by MTS assays. (E) RPMI8226 cells were transfected with antagomir-NC or antagomir-4800, then 48 h later the cells were treated with Bafilomycin A1 for 24 h, and cell inhibition rate was tested by MTS assays. (F,G) The transfected RPMI8226 cells with antagomir-NC or antagomir-4800 were treated with 3MA or Bafilomycin A1, for 24 h, then the cells were harvested for Western blot analysis. **P < 0.01 and ***P < 0.001.