Figure 5.

S1PR signaling pathway regulated PI3K/Akt/mTOR through piR-004800 in MM cells. (A) RPMI8226 and U266 cells were transfected with antagomir-NC or antagomir-4800, 48 h later Western blot analysis was explored. (D) RPMI8226 and U266 cells were transfected with antagomir-NC or antagomir-4800, 48 h later the cells were treated with S1P for 24 h, then Western blot was explored. (B,C,E,F) Relatively quantitative results was determined by Image J and shown as histogram. *P < 0.05, **P < 0.01, and ***P < 0.001.