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. 2020 Mar 24;74(2):441–448. doi: 10.3233/JAD-190948

Fig.1.

Fig.1

Gene and protein expression of autophagy markers LC3B and Cathepsin D in AD, FTD, LBD, and control brains. Gene expression of autophagosome marker LC3B (Hs00797944_s1), lysosomal hydrolase Cathepsin D (CTSD, Hs00157205_m1) and endogenous control Glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Hs03929097_g1) was conducted on cDNA synthesized from homogenates of frontal cortex (F) and hippocampus (H) regions of postmortem brain tissues from AD, LBD, FTD, and healthy controls (Cont) using Taqman assays on the QuantStudio 12K system. Fold change in gene transcripts of LC3B (A) and CTSD (B) was measured using comparative Ct method with GAPDH as endogenous control and Cont-F as the reference biological group. No significant changes were observed in the expression of LCB and Cathepsin D between the different groups. Protein levels of LC3B isoforms LC3I (C) and LC3II (D) and mature isoform of Cathepsin D (E) was also measured using western blotting analysis of the brain homogenates. A representative image of the western blot is shown here (F). No significant change in levels of LC3I (16 kDa) and LC3II (14 kDa) were observed between the different groups. Levels of mature Cathepsin D (30 kDa) was increased in AD-F and LBD-F regions as compared to Cont-F (Mean±SD, *p<0.05).