Fig. 5.
Changes in ST6Gal1 expression leads to broad liver transcriptional change. (A) RNA-seq analysis from young 8–12-week-old ST6Gal1f/f or H-cKO mouse liver samples, showing all 214 differentially expressed genes identified. (B) Volcano plot labeled with the top 50 differentially expressed genes. (C) Volcano plot of genes implicated in the synthesis of N-glycans, showing a distinct lack of change. (D–K) Gene set enrichment analysis (GSEA) confirmed a lack of change in glycosylation (D), but a stronger than expected association and enrichment with several metabolic pathways, including mTOR (E), glycolysis (F), fatty acid metabolism (G), processing of xenobiotic compounds (H) and peroxisome associated genes (I). Two immune pathways, TGFβ (J) and complement protein expression (K) are likewise associated.