Fig. 6.
High fat diet leads to fatty liver in both ST6Gal1f/f and H-cKO mice. (A) Histological analysis via H&E and Oil Red O demonstrates severe fat accumulation in both the ST6Gal1f/f and H-cKO mice on HFD. (B–C) Male mice on HFD gained a considerable (~80%) more weight than their control diet counterparts, to about 200% their initial weight. Female mice on HFD gained about 150% of their initial weight. Weight gain for either group was not dependent on genotype. (D) HFD mice ate approximately 50% more calories than control diet mice. (E–F) Resting blood glucose measurements for male and female mice were not significant impacted by genotype or HFD. Male (G) but not female (H) HFD mice showed significant glucose intolerance in fasting mice challenged with glucose. Mice were approximately 23–26 weeks of age at harvest. AUC, Student’s T test, *P < 0.05.