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Western blot analysis of AMPK and mTOR signaling in older mice. Activation of AMPK (A), ACC (B), RpS6 (C), and 4eBP1 (D) expressed as ratios of phosphorylated to total protein content (P/T). ^Significant effect of sex. *Significantly different from male within treatment. n = 6 per group. All values expressed as mean ± SEM.
