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. Author manuscript; available in PMC: 2021 Feb 6.
Published in final edited form as: Mol Cell. 2020 Jan 23;77(3):645–655.e7. doi: 10.1016/j.molcel.2020.01.003

Figure 4: Iron-mediated rescue of cell proliferation is independent of signaling and metabolite changes associated with lysosomal acidity.

Figure 4:

(A) Comparison of metabolite abundance in 293T whole cells or purified lysosomes upon treatment with BafAl (10nM) in the presence or absence of iron supplementation (FAC 0.4 mg/ml). (Lysosomes r= 0.996, p<0.001; Whole cell r=0.785, P<0.001)

(B) Iron release from transferrin depends on lysosomal acidity. 293T lysates following uptake of biotinylated-holotransferrin, after 24-hour control, BafA1(10nM), or BafAl and FAC (0.1mg/ml) treatments were immobilized on PDVF membranes. Immunoblotting for TF, TFRC, and vinculin loading controls or incubation of membrane with HRP-streptavidin

(C) Immunoblotting for LC3B-II accumulation as an indicator of inhibition of autophagy completion in cells grown under BafA1 (10nM) or FAC (0.4mg/ml) for 24 hours.