TABLE 1.

Polar lipid content and composition from Saccharomyces cerevisiae strain Y02431 (slc4Δ) expressing LPCATs from sunflower in the absence or presence of expression of a FATTY ACID DESATURASE (FAD2) from Camelina sativa.

		Total content			Mole %
		(μ g/ml culture)	CL	PE	PC	PS	PI
CsFAD2−	p423GPD p416GPD	23.3 ± 7.2	1.3 ± 0.6	14.1 ± 3.0	54.4 ± 2.5	5.8 ± 0.9	24.4 ± 5.7
	p423GPD:HaLPCAT1 p416GPD	33.1 ± 4.5	2.1 ± 1.2	15.0 ± 1.9	53.7 ± 2.1	6.1 ± 1.8	23.2 ± 3.4
	p423GPD:HaLPCAT2 p416GPD	32.2 ± 5.9	1.0 ± 0.2	11.5 ± 1.4	47.1 ± 1.7*	7.9 ± 2.6	32.5 ± 1.9*
	p423GPD:HaLPCAT3 p416GPD	24.4 ± 5.1	1.4 ± 0.4	20.5 ± 1.1*	52.9 ± 1.3	4.3 ± 0.4	20.9 ± 2.1
CsFAD2+	p423GPD p416GPD:CsFAD2	29.3 ± 8.5	1.0 ± 0.4	15.0 ± 4.1	48.4 ± 5.7	5.1 ± 1.2	30.5 ± 9.3
	p423GPD:HaLPCAT1 p416GPD:CsFAD2	39.0 ± 13.7	1.1 ± 0.4	12.3 ± 2.7	48.2 ± 3.5	4.9 ± 0.7	33.6 ± 3.0
	p423GPD:HaLPCAT2 p416GPD:CsFAD2	29.9 ± 13.1	1.2 ± 0.6	15.7 ± 2.9	60.9 ± 4.9*	5.1 ± 0.6	17.1 ± 2.9*
	p423GPD:HaLPCAT3 p416GPD:CsFAD2	27.9 ± 3.3	1.9 ± 0.8	19.5 ± 0.7	53.9 ± 2.4	4.3 ± 0.3	20.3 ± 1.3

HaLPCATs were expressed in the vector p423GPD, whereas CsFAD2 was expressed in p416GPD. Control lines correspond to empty plasmids. Data are the average of four independent determinations ± standard deviation. Asterisks indicate significant differences with control cells according to one-way ANOVA with Tukey post hoc analysis with a significance level of 0.05%. CL: cardiolipin; PE: phosphatidylethanolamine; PC: phosphatidylcholine; PS: phosphatidylserine; PI: phosphatidylinositol.