TABLE 2.
Fatty acid composition of triacylglycerols (TAG), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) from Saccharomyces cerevisiae strain Y02431 (slc4Δ) expressing LPCATs from sunflower in the presence or absence of expression of Camelina sativa FATTY ACID DESATURASE 2 (CsFAD2).
| Composition (mole%) | ||||||||||
| CsFAD2− | 16:0 | 16:1Δ9 | 16:2Δ9,12 | 18:0 | 18:1Δ9 | 18:1Δ11 | 18:2Δ9,12 | 18:2Δ11,14 | ||
| PC | Control | 38.6 ± 5.3 | 21.2 ± 2.8 | nd | 13.3 ± 2.0 | 19.7 ± 3.5 | 7.2 ± 2.3 | nd | nd | |
| HaLPCAT1 | 24.9 ± 1.4* | 31.6 ± 1.8* | nd | 11.3 ± 1.4 | 27.6 ± 1.4* | 4.6 ± 1.1* | nd | nd | ||
| HaLPCAT2 | 61.1 ± 1.3* | 8.1 ± 1.4* | nd | 13.3 ± 2.0 | 12.9 ± 2.0 | 4.7 ± 4.3* | nd | nd | ||
| HaLPCAT3 | 28.4 ± 5.7 | 26.9 ± 6.1 | nd | 15.9 ± 3.7 | 24.2 ± 3.8 | 4.7 ± 1.2* | nd | nd | ||
| PE | Control | 45.5 ± 0.4 | 18.4 ± 4.8 | nd | 12.8 ± 8.5 | 18.3 ± 1.8 | 4.9 ± 1.5 | nd | nd | |
| HaLPCAT1 | 47.9 ± 4.1 | 21.0 ± 1.7 | nd | 13.3 ± 4.7 | 15.2 ± 2.9 | 2.7 ± 0.3* | nd | nd | ||
| HaLPCAT2 | 61.9 ± 1.3* | 8.1 ± 1.4* | nd | 13.5 ± 2.0 | 14.4 ± 2.0 | 2.3 ± 1.1* | nd | nd | ||
| HaLPCAT3 | 42.6 ± 3.9 | 17.6 ± 4.4 | nd | 22.1 ± 9.4 | 15.0 ± 1.8 | 2.8 ± 0.4* | nd | nd | ||
| TAG | Control | 20.9 ± 1.0 | 42.8 ± 1.4 | nd | 9.2 ± 0.4 | 19.1 ± 1.5 | 8.0 ± 1.3 | nd | nd | |
| HaLPCAT1 | 17.1 ± 3.4 | 52.0 ± 5.3* | nd | 6.3 ± 1.5 | 20.3 ± 2.2 | 4.4 ± 0.9* | nd | nd | ||
| HaLPCAT2 | 23.1 ± 7.4 | 41.1 ± 4.7 | nd | 9.5 ± 2.1 | 22.0 ± 4.5 | 4.3 ± 1.1* | nd | nd | ||
| HaLPCAT3 | 19.8 ± 2.0 | 42.6 ± 0.7 | nd | 10.9 ± 0.8 | 22.0 ± 2.1 | 4.6 ± 1.0* | nd | nd | ||
| CsFAD2+ | 16:0 | 16:1Δ9 | 16:2Δ9,12 | 18:0 | 18:1Δ9 | 18:1Δ11 | 18:2Δ9,12 | 18:2Δ11,14 | Total PUFA | |
| PC | Control | 45.1 ± 8.1 | 15.7 ± 4.9 | 2.2 ± 0.9 | 20.2 ± 2.4 | 6.9 ± 1.7 | 1.8 ± 0.5 | 7.8 ± 3.5 | 0.3 ± 0.1 | 10.4 ± 3.6 |
| HaLPCAT1 | 33.8 ± 3.8* | 21.9 ± 6.6 | 3.5 ± 1.4 | 19.5 ± 5.3 | 7.1 ± 2.0 | 1.5 ± 0.5 | 12.1 ± 3.2 | 0.5 ± 0.2 | 16.1 ± 3.5 | |
| HaLPCAT2 | 51.6 ± 4.9 | 8.7 ± 4.0 | 0.8 ± 0.2 | 28.4 ± 2.5 | 5.7 ± 2.8 | 1.3 ± 0.6 | 3.3 ± 0.2 | 0.2 ± 0.0 | 4.2 ± 0.3 | |
| HaLPCAT3 | 31.6 ± 1.3* | 25.4 ± 1.5* | 2.5 ± 1.0 | 16.5 ± 0.8 | 9.0 ± 1.9 | 4.6 ± 0.4* | 10.0 ± 0.6 | 0.5 ± 0.1 | 13.0 ± 1.2 | |
| PE | Control | 76.3 ± 4.2 | 5.5 ± 2.3 | 0.0 ± 0.0 | 14.5 ± 2.7 | 3.3 ± 0.3 | 0.0 ± 0.0 | 0.4 ± 0.1 | 0.0 ± 0.0 | 0.4 ± 0.1 |
| HaLPCAT1 | 50.8 ± 6.1* | 16.5 ± 2.4* | 1.5 ± 0.4* | 14.3 ± 2.2 | 6.4 ± 2.4 | 0.5 ± 0.7 | 10.0 ± 1.1* | 0.0 ± 0.0 | 11.5 ± 1.2* | |
| HaLPCAT2 | 72.5 ± 4.4 | 6.2 ± 1.8 | 0.0 ± 0.0 | 16.3 ± 2.5 | 2.7 ± 3.4 | 0.5 ± 0.7 | 1.8 ± 1.2 | 0.0 ± 0.0 | 1.8 ± 1.2 | |
| HaLPCAT3 | 53.1 ± 4.0* | 21.2 ± 2.1* | 0.4 ± 0.1* | 7.9 ± 2.7* | 11.7 ± 3.6* | 1.9 ± 0.7* | 3.8 ± 0.6* | 0.0 ± 0.0 | 4.2 ± 0.6* | |
| TAG | Control | 20.8 ± 3.8 | 37.5 ± 4.5 | 5.5 ± 1.4 | 9.2 ± 1.5 | 11.5 ± 1.8 | 3.2 ± 1.0 | 11.6 ± 2.4 | 0.6 ± 0.3 | 17.8 ± 2.8 |
| HaLPCAT1 | 18.5 ± 3.2 | 32.6 ± 5.3 | 8.0 ± 2.3 | 10.3 ± 1.1 | 9.5 ± 1.8 | 1.9 ± 0.5* | 18.2 ± 3.8* | 1.0 ± 0.4 | 27.2 ± 4.4* | |
| HaLPCAT2 | 22.7 ± 3.7 | 35.0 ± 7.2 | 4.6 ± 2.0 | 12.1 ± 3.8 | 11.0 ± 2.8 | 3.1 ± 0.8 | 10.9 ± 1.1 | 0.5 ± 0.2 | 16.1 ± 2.3 | |
| HaLPCAT3 | 19.0 ± 2.2 | 36.9 ± 4.2 | 6.2 ± 1.5 | 9.9 ± 1.5 | 9.2 ± 0.9 | 4.8 ± 1.3 | 13.1 ± 1.4 | 0.9 ± 0.2 | 20.2 ± 2.1 | |
Data are the average of four independent determinations ± standard deviation. Asterisks indicate significant differences with control cells according to one-way ANOVA with Tukey post hoc analysis with a significance level of 0.05%. nd: not detected.