Fig. 2.

hCD81 variants and WT hCD81 translocate to the cell surface of human hepatoma cells. a Immunoblot of cell lysates from Lunet N#3 cells transduced with lentiviral pseudoparticles harboring the different hCD81 variants, WT hCD81 or control vector and probed with anti-CD81 antibody. GAPDH served as loading control and Lunet N#3 cells as negative control. b Cell surface expression of hCD81 assessed by flow cytometry after staining with anti-hCD81-APC antibody. An APC-conjugated isotype control antibody or buffer only served as negative controls. Representative histograms comparing anti-hCD81-APC-stained, isotype control-stained and unstained cells from all cell lines included in this study. The histograms show one out of two independent experiments with 2.0 × 10⁴ cells per measurement. The gate for hCD81-positive cells is shown as horizontal line. Representative histograms of two independent biological replicates are shown