Fig. 5.

A subset of hCD81 variants reduces HCV susceptibility of human hepatoma cells. hCD81-variant expressing cells were challenged for 4 h with cell culture-derived HCV chimeras encoding the glycoproteins of the seven indicated genotypes as well as Renilla luciferase. Luciferase activity in cell lysates was measured 72 h post-infection and the results were plotted relative to infection of cells expressing WT CD81. Mean + SD of three independent biological replicates each performed in technical triplicates shown (GT5a and F186A variant experiments performed twice in technical triplicates). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001