Table 4.

Sensitivity and specificity of kits tested at 3 laboratories compared to confirmed reference results.

Overall CDC Diagnosis			Laboratory results
			ADB-DRL				PHAC				MPIR				Combined
Test	Measure	N	POS	EQ	NEG	%	POS	EQ	NEG	%	POS	EQ	NEG	%	% (95% CI)
InBiosa	Sensitivity	64	54b	NA	9	84.4	52b	NA	11	81.3	53b	NA	11	82.8	82.8 (77.0, 87.7)
	Specificityc	78	14b	NA	57	73.1	6b	NA	71	91.0	10b	NA	67	87.2	83.3 (78.0, 87.6)
	Specificityd	155	45b	NA	110e	71.0	40b	NA	115d	74.2	42b	NA	113e	72.9	72.7 (68.5,76.6)
NovaTec	Sensitivityf	64	40	3	21	67.2	40	5	19	70.3	45	1	18	71.9	69.8 (63.1, 76.0)
	Specificityc	78	2	0	76	97.4	1	0	77	98.7	2	0	76	97.4	97.9 (95.5, 99.2)
	Specificityd	155	3	0	152	98.1	2	0	153	98.7	6	0	149	96.1	97.6 (96.0, 98.8)
Euroimmun IgM	Sensitivitye	64	28	4	32	50.0	30	3	31	51.6	33	4	27	57.8	53.1 (46.1, 60.1)
	Specificityc	78	0	1	77	98.7	1	1	76	97.4	3	1	74	96.2	97.0 (94.3, 98.7)
	Specificityd	155	2	1	152	98.1	2	1	152	98.1	5	1	149	96.8	97.4 (95.7, 98.6)
Euroimmun IgG	Sensitivityf	64	20	1	43	32.8	20	0	44	31.3	22	3	39	39.1	34.4 (27.9, 41.3)
	Specificityc	78	1	0	77	98.7	0	1	77	98.7	2	0	76	97.4	98.3 (96.1, 99.5)
	Specificityd	155	5	2	148	95.5	5	3	147	94.8	9	3	143	94.2	94.2 (91.8, 96.1)
Euroimmun IgM + IgG	Sensitivityf	64	39	NA	25	60.9	39	NA	25	60.9	42	NA	22	65.6	62.5 (55.5, 69.1)
	Specificityc	78	1	NA	77	98.7	1	NA	77	98.7	5	NA	73	93.6	97.0 (94.3, 98.7)
	Specificityd	155	7	NA	148	95.5	7	NA	148	95.5	14	NA	141	91.0	94.0 (91.6, 95.9)

^aFor InBios, positive plus negative numbers do not add up to N in some cases, because of the “Presumptive Other Flavivirus” category.

^bFor the InBios kit, positive results are considered both “Presumptive Zika positive” and “Possible Zika positive”.

^cSpecificity denominator consists of true negative samples only.

^dSpecificity denominator consists of 10 YF, 10 WN, 10 CHIK, 47 DEN and 78 NEG samples.

^eNegative plus Presumptive Other Flavivirus results are classified as a negative result.

^fSensitivity was calculated where equivocal results were classified as positive.