TABLE 1.
Volumes, areas, and hiPSC plating densities for different Laminin-coated plate types.
| Plate type | Well area | Coating volume | Rinse volume | Versene/ TrypLE-Sel. volume | large fragments (≈200–400 cells) | small clumps (≈5–50 cells) | single cell-dissociated | Medium feed volume |
|---|---|---|---|---|---|---|---|---|
| 96w | 0.34 cm2 | 70 μl | 200 μl | 100 μl | 9 | 90 | 900–10,000 | 150 μl |
| 24w | 1.9 cm2 | 300 μl | 700 μl | 450 μl | 35 | 350 | 5–57,000 | 600 μl |
| 12w | 3.5 cm2 | 500 μl | 1.5 ml | 800 μl | 60 | 600 | 9–105,000 | 1 ml |
| 6w | 9.6 cm2 | 1.0 ml | 3.0 ml | 1.9 ml | 160 | 1,600 | 24–300,000 | 2.5 ml |