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. 2020 Apr 22;15(4):e0231470. doi: 10.1371/journal.pone.0231470

Fig 5. BCL6 co-repressors are expressed and functional in glioblastoma.

Fig 5

A. Cycle thresholds for QRT-PCR for the housekeeping gene HPRT, BCL6 and co-repressors BCOR, NCOR1, and SMRT (NCOR2) in LN18 and U87-MG cells. B. Western Blot for BCOR, NCOR1, SMRT (NCOR2), alpha tubulin (TUBA), and beta actin (ACTB) in LN18, U87-MG, K562 (negative control) and Raji (positive control). C. Western blot for BCL6, alpha tubulin (TUBA), and beta actin (ACTB) of LN18 cells transfected with BCL6wt, GFP or non-transfected (N.T) with K562 and Raji cells as BCL6 negative and positive controls, respectively (left panel). Cells were harvested 48 hours after transfection. Luciferase assay of LN18 cells co-transfected with both BCL64-tkLUC and BCL6wt plasmids or PGL3 and BCL6wt plasmids and harvested 48 hours after transfection (right panel). The luciferase values were first normalized to cell number and then expressed as a proportion of their single transfected control (either BCL64-tkLUC or PGL3 alone).