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. 2020 Apr 22;15(4):e0231870. doi: 10.1371/journal.pone.0231870

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© 2020 Pamphlett et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) A brown-immunostained perivascular astrocyte in the LGN contains particulate mercury in its cytoplasm and processes (box 1). Particulate mercury (arrowhead) is present in the perivascular glia limitans, next to the artefactually-expanded perivascular space (*) of a small blood vessel (BV). Mercury is present adjacent to the nucleus of some oligodendrocytes (eg, box 2) and in the cytoplasm of neurons (eg, box 3). AMG/hematoxylin/GFAP. (B) Numerous perivascular brown-immunostained hyperplastic astrocytes in this LGN contain black mercury deposits (eg, arrow and box C, enlarged in 4C). Most neuronal (eg, circled) and some oligodendrocyte (arrowhead) cell bodies contain mercury. AMG/hematoxylin/GFAP. (C) Enlarged view of box C in (B) shows mercury in the cytoplasm of a hypertrophic astrocyte (open arrow) with processes extending to surrounding mercury-containing neurons (closed arrows) and oligodendrocytes, one with cytoplasmic mercury (circled) and one without (dash circled). AMG/hematoxylin/GFAP. G: identify number.