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. 2020 Mar 5;40(5):1182–1194. doi: 10.1161/ATVBAHA.120.313906

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© 2020 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 1. — Purification of nascent LpA-I (apo AI [apolipoprotein AI] containing particles formed by incubating ABCA1 [ATP-binding cassette transporter 1]-expressing cells with apo AI) particles. A, LpA-I particles were fractionated by size exclusion chromatography on a Superose 6 column. Fractions were collected, pooled as indicated and designated as 1-LpA-I (light gray), 2-LpA-I (black), and 3-LpA-I (dark gray) from the largest to the smallest particle size. B, LpA-I subspecies were separated by nondenaturing polyacrylamide gradient gel electrophoresis and visualized by Coomassie Blue G-250 staining. mAU indicates milli absorbance unit.