TABLE 1.

Primers used for screening of broad-spectrum β-lactamase, ESBL, and AmpC genetic determinants (Dallenne et al., 2010) as well as integron prevalence (de Paula et al., 2018) in selected Enterobacteriaceae isolated from water, fresh produce, and contact surfaces.

Target genes	Primer sequences	Thermocycling conditions	Expected amplicon size (bp)
blaTEM	TEM-F: 5′-CATTTCCGTGTCGCCCTTATTC-3′	94°C, 10 min;	800
	TEM-R: 5′-CGTTCATCCATAGTTGCCTGAC-3′	30 cycles of
blaSHV	SHV-F: 5′-AGCCGCTTGAGCAAATTAAAC-3′	94°C, 40 s,	713
	SHV-R: 5′-ATCCCGCAGATAAATCACCAC-3′	58°C, 40 s,
blaOXA–1like	OXA-F: 5′-GGCACCAGATTCAACTTCAAG-3′	72°C, 1 min;	564
	OXA-R: 5′-GACCCCAAGTTTCCTGTAAGTG-3′	72°C, 7min
blaCTX–M Group8/25	CTX-M Gp8/25-F: 5′-AACRCRCAGACGCTCTAC-3′	94°C, 10 min;	326
	CTX-M Gp8/25-R: 5′-TCGAGCCGGAASGTGTYAT-3′	30 cycles of
blaCTX–M Group 9	CTX-M Gp9-F: 5′-TCAAGCCTGCCGATCTGGT-3′	94°C, 40 s,	688
	CTX-M Gp9-R: 5′-TGATTCTCGCCGCTGAAG-3′	60°C, 40 s,
blaCTX–M Group 1	CTX-M Gp1-F: 5′-TTAGGAARTGTGCCGCTGYA-3′	72°C, 1 min;	561
	CTX-M Gp1-R: 5′-CGATATCGTTGGTGGTRCCAT-3′	72°C, 7 min
blaACC	ACC-F: 5′-CACCTCCAGCGACTTGTTAC-3′ ACC-R: 5′-GTTAGCCAGCATCACGATCC-3′	94°C, 10 min;	346
		30 cycles of
		94°C, 40 s,
		60.5°C, 40 s,
		72°C, 1 min;
		72°C, 7 min
blaFOX	FOX-F: 5′-CTACAGTGCGGGTGGTTT-3′	94°C, 10 min;	162
	FOX-R: 5′-CTATTTGCGGCCAGGTGA-3′	30 cycles of
blaMOX	MOX-F: 5′-GCAACAACGACAATCCATCCT-3′	94°C, 40 s,	895
	MOX-R: 5′-GGGATAGGCGTAACTCTCCCAA-3′	59.6°C, 40 s,
blaDHA	DHA-F: 5′-TGATGGCACAGCAGGATATTC-3′	72°C, 1 min;	997
	DHA-R: 5′-GCTTTGACTCTTTCGGTATTCG-3′	72°C, 7 min
blaCIT	CIT-F: 5′-CGAAGAGGCAATGACCAGAC-3′		538
	CIT-R: 5′-ACGGACAGGGTTAGGATAGY-3′
blaEBC	EBC-F: 5′-CGGTAAAGCCGATGTTGCG-3′		683
	EBC-R: 5′-AGCCTAACCCCTGATACA-3′
IntI1	Int1-F: 5′-GGT CAAGGATCTGGATTTCG-3′	94°C, 12 min;	436
	Int1-R: 5′-ACATGCGTGTAAATCATCGTC-3′	30 cycles of
IntI2	Int2-F: 5′-CACGGATATGCGACAAAAAGG-3′	94°C, 30 s,	788
	Int2-R: 5′-TGTAGCAAACGAGTGACGAAATG-3′	60°C, 30s,
IntI3	Int3-F: 5′-AGTGGGTGGCGAATGAGTG-3′	72°C, 1 min;	600
	Int3-R: 5′-TGTTCTTGTATCGGCAGGTG-3′	72°C, 8 min