FIGURE 3.

Lactobacillus reuteri LMG P-27481 hydrogen peroxide release and adherence to human HT-29 monolayers. (A) Production of H₂O₂ by probiotics expressed as μg/ml of metabolite released by 10⁹ viable CFUs after 24 h of incubation. (B) Antibiotic resistance image obtained by CARD showed the presence of nonperfect and strict and 111 loose hits. (C) Confluent HT-29 monolayers were washed and incubated with the probiotic strain (MOI 1:10) for 1 h at 37°C. To quantify adhering bacterial cells, the monolayers were extensively washed, lysed and aliquots seeded on MRS agar plates. Following 48 h of incubation in anaerobiosis, the colonies were enumerated and adhering bacteria expressed as the percentage of the total initial population of viable probiotic cells (n = 3 different experiments in duplicate). Data are presented as mean ± SD.