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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Anal Chem. 2019 Dec 6;91(24):15370–15376. doi: 10.1021/acs.analchem.9b03536

Figure 1.

Figure 1.

MALDI-MSI-PBC workflow. First, wax patterns were printed onto Whatman 105 lens paper. Next, the zones of the patterned scaffolds were seeded with either cell-laden Matrigel or Matrigel only. After an overnight incubation in culture medium, the scaffolds were dried using a vacuum centrifuge and then mounted onto a MALDI target plate with double-sided conductive tape. Next, the drug solutions were spotted onto selected wells and allowed to redry. Matrix was then applied using a robotic sprayer. Finally, the scaffolds were analyzed with MALDI-MSI to generate the ion density maps.