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. Author manuscript; available in PMC: 2021 Mar 3.
Published in final edited form as: Structure. 2020 Jan 28;28(3):378–384.e4. doi: 10.1016/j.str.2020.01.004

Figure 3. Hierarchy in tubulin occupancies in MTBR.

Figure 3

(A) Equivalent residues tested highlighted in red, orange, blue and grey for R1 – R4 respectively. Normalized Δλmax of tauacrylodan at (B) K281 and (C) D283-equivalent positions upon binding to tubulin. Tau only measurements were plotted at 1E-4 μM tubulin for visualization. Data points are mean ± SD, n≥3. Insets are the raw Δλmax. Approximate KDs from binding to [tubulin] <2 μM are reported below. (D and E) Bar graph for Δλmax with 2 or 10 μM tubulin for comparison.