Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) |
Nudt21 | NCBI Gene ID: 68219 | ||
| Gene (Homo sapiens) |
NUDT21 | NCBI Gene ID: 11051 | ||
| Strain, strain background (M. musculus; male and female) | C57BL/6J | The Jackson Laboratory | IMSR Cat# JAX:000664, RRID:IMSR_JAX:000664 | |
| Genetic reagent (M. musculus) | Sox2-Cre | PMID:14595839 | IMSR Cat# JAX:008454, RRID:IMSR_JAX:008454 | |
| Genetic reagent (M. musculus) | Nudt21flox/flox | PMID:30830875 | RRID:MGI:6385511 | Dr. Eric Wagner (University of Texas Medical Branch) |
| Genetic reagent (M. musculus) | Nudt21+/- | This paper | RRID:MGI:6385902 | Line maintained in the H. Zogbhi lab |
| Cell line (H. sapiens) | HEK293T | ATCC | ECACC Cat# 12022001, RRID:CVCL_0063 | |
| Cell line (H. sapiens) | WA09 | WiCell | RRID:CVCL_9773 | |
| Antibody | Anti-CFIm25 (rabbit polyclonal) | Proteintech | Proteintech Cat# 10322–1-AP, RRID:AB_2251496 | 1:50 |
| Antibody | Anti-NeuN (mouse monoclonal) | Millipore | Millipore Cat# MAB377, RRID:AB_2298772 | 1:50 |
| Antibody | Anti-CFIm25 (mouse monoclonal) | Santa Cruz Biotechnology | Santa Cruz Biotechnology Cat# sc-81109, RRID:AB_2153989 | 1:500 |
| Antibody | Anti-H3 (rabbit monoclonal) | Cell Signaling Technology | Cell Signaling Technology Cat# 4499, RRID:AB_10544537 | 1:10,000 |
| Recombinant DNA reagent | pGIPZ | Open Biosystems | shRNA backbone | |
| Sequence-based reagent | Nudt21 forward | This paper | qPCR primer | TACATCCAGCAGACCAAGCC |
| Sequence-based reagent | Nudt21 reverse | This paper | qPCR primer | AATCTGGCTGCAACAGAGCT |
| Sequence-based reagent | Gapdh forward | This paper | qPCR primer | GGCATTGCTCTCAATGACAA |
| Sequence-based reagent | Gapdh reverse | This paper | qPCR primer | CCCTGTTGCTGTAGCCGTAT |
| Sequence-based reagent | NUDT21 forward | This paper | qPCR primer | CTTCAAACTACCTGGTGGTG |
| Sequence-based reagent | NUDT21 reverse | This paper | qPCR primer | AAACTCCATCCTGACGACC |
| Sequence-based reagent | GAPDH forward | This paper | qPCR primer | CGACCACTTTGTCAAGCTCA |
| Sequence-based reagent | GAPDH reverse | This paper | qPCR primer | TTACTCCTTGGAGGCCATGT |
| Sequence-based reagent | Nudt21null forward | This paper | PCR primer | ACAGATTAGCTGTTAGTACAGG |
| Sequence-based reagent | Nudt21null reverse | This paper | PCR primer | GAAGAACCAGAGGAAACGTGAG |
| Sequence-based reagent | Wild-type Nudt21 forward | This paper | PCR primer | AGGAGGCTGACATGGATTGTT |
| Sequence-based reagent | Wild-type Nudt21 reverse | This paper | PCR primer | TCTTCTCCTGGGTTAAGTTCCC |
| Sequence-based reagent | Anti-NUDT21 shRNA 1 | Dharmacon | Clone ID: V2LHS_253272 | CAACCTGTACCCTCTTACCAATTATAC |
| Sequence-based reagent | Anti-NUDT21 shRNA 2 | Dharmacon | Clone ID: V2LHS_197948 | ATCCATATATTCCTGCACATATT |
| Sequence-based reagent | Non-silencing shRNA | Dharmacon | Clone ID: RHS4348 | |
| Sequence-based reagent | P7 adapter (Illumina_4N_21T) | Illumina | Reverse transcription primer | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNTTTTTTTTTTTTTTTTTTTTT |
| Sequence-based reagent | P5 adapter | Interated DNA Technologies | Reverse transcription primer | 5′HexynylNNNNAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT |
| Sequence-based reagent | Universal primer | cDNA amplification primer | AATGATACGGCGACCACCGAG | |
| Sequence-based reagent | Example 3′ indexing primer | cDNA amplification primer | CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCAGACGTGT | |
| Sequence-based reagent | Ddx6 forward | This paper | qPCR primer | TGGATCTCATCAAGAAAGGC |
| Sequence-based reagent | Ddx6 reverse | This paper | qPCR primer | GTGACAACAATTTATCTGCCTC |
| Sequence-based reagent | Ddx6-long forward | This paper | qPCR primer | CACAGCTGACAGACTCCAACA |
| Sequence-based reagent | Ddx6-long reverse | This paper | qPCR primer | AGCTTACTAACCCAGGCCCA |
| Sequence-based reagent | MECP2 forward | This paper | qPCR primer | GATCAATCCCCAGGGAAAAGC |
| Sequence-based reagent | MECP2 reverse | This paper | qPCR primer | CCTCTCCCAGTTACCGTGAAG |
| Sequence-based reagent | MECP2-long forward | This paper | qPCR primer | GCCTGGAAACCTGTCTGAGG |
| Sequence-based reagent | MECP2-long reverse | This paper | qPCR primer | CTCCAGCTAAGTGTGTCCCG |
| Sequence-based reagent | VMA21 forward | This paper | qPCR primer | TACATATTTGAAGGCGCCC |
| Sequence-based reagent | VMA21 reverse | This paper | qPCR primer | CATACACAAAGAGGGCCAG |
| Sequence-based reagent | VMA21-long forward | This paper | qPCR primer | AGGGGGAGGATTTGGATGTG |
| Sequence-based reagent | VMA21-long reverse | This paper | qPCR primer | TAGCTAAAGAACTCAAGCCCCC |
| Sequence-based reagent | PAK1 forward | This paper | qPCR primer | GAATTACTTGGACAGTTACCTCG |
| Sequence-based reagent | PAK1 reverse | This paper | qPCR primer | ACATCTGTCAAGGAGCCTC |
| Sequence-based reagent | PAK1-long forward | This paper | qPCR primer | CCAGCATTGTGGCTTGTCAT |
| Sequence-based reagent | PAK1-long reverse | This paper | qPCR primer | TTGTGCTGCAGAGGCAGTAG |
| Commercial assay or kit | QuantiTect Reverse Transcription Kit | Qiagen | Cat# 205313 | |
| Chemical compound, drug | 5-Fluoro-2′-deoxyuridine | Sigma-Aldrich | Product Number: F 0503 | 1 µM |
| Software, algorithm | LI-COR Image Studio | LI-COR | Image Studio Lite, RRID:SCR_013715 | |
| Software, algorithm | FreezeFrame 3 | Actimetrics | RRID:SCR_014429 | Conditioned fear assay |
| Software, algorithm | Fusion | Accuscan Instruments | RRID:SCR_017972 | Open field assay |
| Software, algorithm | EthoVision XT | Noldus Information Technology | RRID:SCR_000441 | Morris water maze |
| Software, algorithm | Clampfit 10 | Molecular Devices | RRID:SCR_011323 | EEG |
| Software, algorithm | Proteome Discoverer | Thermo Fisher Scientific | RRID:SCR_014477 | Mass spectrometry |
| Software, algorithm | Prism | Graphpad | RRID:SCR_002798 |