Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Marivirga tractuosa)
|
MtTMEM175 |
DSM 4126 | E4TN31 | |
| Gene (Homo sapiens) |
hTMEM175 |
Sourcebioscience |
Q9BSA9, IRAUp969F1019D |
|
| Gene (Streptomyces collinus) | scTMEM175 |
Synthesized by GenScript |
S5VBU1 |
|
| Recombinant DNA reagent |
pBXC3H |
Dutzler lab |
Addgene # 47068 |
|
| Recombinant DNA reagent |
pcDXC3M |
Dutzler lab |
Addgene #49030 |
|
| Recombinant DNA reagent |
pcDXC3GMS |
Dutzler lab |
Addgene #49031 |
|
| Recombinant DNA reagent |
pBXNPHM3 |
Dutzler lab |
Addgene #110099 |
|
| Cell line (Homo sapiens) |
HEK293 | Germ. Collection Microorg. & cell cultures, Braunschweig, Germany |
ACC 305, tested mycoplasma negative |
|
| Strain, strain background (Escherichia coli) |
MC1061 |
Dutzler lab |
Made by Malcolm Casadaban https://cgsc.biology.yale.edu/Strain.php?ID=11225 |
|
| Commercial assay or kit |
Superdex 200 10/300 |
GE healthcare |
Cat#17517501 |
|
| Commercial assay or kit |
Pierce Streptavidin Plus UltraLink resin |
Thermo Fisher Scientific |
Cat#53117 |
|
| Commercial assay or kit |
Ni-NTA resin |
Qiagen |
Cat#30230 |
|
| Commercial assay or kit |
Superdex 200 5/150 increase | GE healthcare |
Cat#28990945 |
|
| Commercial assay or kit |
Strep-Tactin Superflow high capacity 50% suspension | Iba lifesciences |
Cat#2-1208-010 |
|
| Chemical compound, drug |
cOmplete, EDTA-free Protease Inhibitor Cocktail |
Roche |
Cat#5056489001 |
|
| Chemical compound, drug |
n-dodecyl-β-d-maltopyranoside, Solgrade |
Anatrace |
Cat#D310S |
|
| Chemical compound, drug |
n-decyl-β-d-maltopyranoside, Solgrade |
Anatrace |
Cat#D322S |
|
| Chemical compound, drug |
Desthiobiotin |
Iba lifesciences |
Cat#2-1000-002 |
|
| Chemical compound, drug |
E. coli polar lipids |
Avanti polar lipids |
Cat#100600 | |
| Chemical compound, drug |
Fugene |
Promega |
Cat#E2311 |
|
| Chemical compound, drug |
CellMask Deep Red |
Thermo Fisher |
Cat#C10046 |
|
|
Chemical compound, drug |
ER-tracker Blue-white DPX |
Thermo Fisher |
Cat#E12353 |
|
| Chemical compound, drug | Accutase |
Thermo Fischer | Cat#A1110501 | |
| Chemical compound, drug | Dulbecco’s modified Eagle’s medium | Sigma | Cat#D5671 |
|
| Chemical compound, drug | L-Glutamine |
Sigma |
Cat#G7513 |
|
| Chemical compound, drug | GeneJuice Transfection Reagent | Millipore Corp |
Cat#70967-5 | |
| Chemical compound, drug | Fetal bovine serum (FBS) | Sigma | Cat#BCBV7601 and Cat#F7524 | |
| Other | EPC-9 Amplifier | Heka Electronics | ||
| Software, algorithm | Fiji | Schindelin et al., 2012 | http://imagej.net/Fiji | |
| Software, algorithm | PulseFit | Heka Electronics | ||
| Software, algorithm | PatchMaster V2x90,3 | Heka Electronics | ||
| Software, algorithm | FitMaster V2x90,1 | Heka Electronics | ||
| Software, algorithm | IGOR version 6.3.7.2 | WaveMetrics | ||
| Software, algorithm | JPCalcWin version 1.01 | https://medicalsciences.med.unsw.edu.au/research/research-services/ies/jpcalcwin |