Table 1.
Annotations of the alanosine (ala) biosynthetic gene cluster. The nucleotide sequences have been deposited into the NCBI database (Genbank accession numbers for AlaA-L are MN603934-MN603945).
| Protein | Size (aa) | Predicted Function | Closest Homolog | Accession # | %Identity/ %Similarity |
|---|---|---|---|---|---|
| AlaA | 324 | 2,3-diaminopropionate biosynthesis protein | SbnA, Streptomyces hirsutus | WP_055594931.1 | 87/91 |
| AlaB | 353 | 2,3-diaminopropionate biosynthesis protein | SbnB, Streptomyces hirsutus | WP_055594930.1 | 84/89 |
| AlaC | 557 | amino acid adenylation domain-containing protein | Streptomyces hirsutus | WP_055594929.1 | 79/84 |
| AlaD | 385 | acyl-CoA dehydrogenase | Streptomyces hirsutus | WP_055594947.1 | 89/94 |
| AlaE | 190 | hypothetical protein | Streptomyces hirsutus | WP_055594928.1 | 80/88 |
| AlaF | 237 | thioesterase | hypothetical protein, Streptomyces hirsutus | WP_079034952.1 | 76/83 |
| AlaG | 286 | hypothetical protein | Streptomyces hirsutus | WP_055594925.1 | 86/92 |
| AlaH | 177 | NADPH-dependent FMN reductase | Streptomyces hirsutus | WP_055594946.1 | 87/92 |
| AlaI | 657 | FAD/NAD(P)-binding protein | FAD/NAD(P)-binding protein [Streptomyces kanamyceticus] | WP_055544225.1 | 76/82 |
| AlaJ | 470 | nitro-succinate lyase | 3-carboxy-cis,cis-muconate cycloisomerase [Streptomyces sp. BK161] | TDT22211.1 | 75/80 |
| AlaK | 273 | SARP family regulator | CreF [Streptomyces cremeus] | ALA99205.1 | 57/69 |
| AlaL | 79 | carrier protein | MULTISPECIES: [Streptomyces] | WP_030834819.1 | 91/79 |