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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Mol Microbiol. 2020 Jan 6;113(4):807–825. doi: 10.1111/mmi.14445

Figure 1. Polar lipids of A. finegoldii.

Figure 1.

PE, phosphatidylethanolamine; SL, sulfonolipid; U1 and U2 were not identified; NL, neutral lipids. PE and SL were identified by mass spectrometry analysis as shown in subsequent figures.

A. A. finegoldii was labeled with [14C]acetate and the lipid extract was separated by two-dimensional chromatography on Silica Gel H thin layers using chloroform:methanol:acetic acid:water (80/25/10/2, v/v) in the first dimension followed by diisobutylketone:acetic acid:water (80/55/15, v/v) in the second dimension. The labeled lipids were visualized with a PhosphorImager system.

B. Lipid extracts from cells labeled with either [14C]acetate (A) or [14C]oleate (O) were separated using the first-dimension solvent system.

C. Separation of [14C]acetate (A) or [14C]oleate (O) labeled lipids using the second-dimension solvent system.