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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Mol Microbiol. 2020 Jan 6;113(4):807–825. doi: 10.1111/mmi.14445

Figure 3. A. finegoldii growth and FASII essentiality.

Figure 3.

A. The growth of A. finegoldii was monitored in either DSM Medium 104 (104, red), DSM Medium 104 excluding Tween 80 (104-T, blue), or lipid-free WCAB defined medium (WCAB, black). Growth was monitored over three days, and data is presented as the mean A600 ± S.E.M. of three independent cultures.

B. Concentration-dependent growth inhibition of A. finegoldii by the FASII inhibitor cerulenin in 104-T medium (blue), in 104-T medium supplemented with 50 μM anteiso-15:0 (green), or 104-T medium supplemented with 50 μM 18:1Δ9 (purple). A. finegoldii was back-diluted 1:100 from a stationary phase stock culture grown in 104-T medium into 104-T medium containing 1% v/v DMSO, and the indicated concentrations of cerulenin and the A600 of the cultures was measured at 48 hours. Growth data is the mean A600 ± S.E.M. of three biological replicates.