Figure 8. Acyl carrier and chain length selectivity of AfPlsC.

E. coli strain SM2–1 (plsC(Ts)) was complemented with plasmids expressing either EcPlsC or AfPlsC and grown at the non-permissive temperature (42°C). The PE molecular species were determined to assess AfPlsC acyl carrier and acyl chain selectivity. The insets show the fragmentation of a relevant peak in the spectrum to confirm the identity and positional distribution of the fatty acids. The spectra shown are representative of three independent experiments.

A. PE molecular species profile of E. coli strain SM2-1 complemented with EcPlsC.

B. PE molecular species profile of E. coli strain SM2-1 complemented with AfPlsC.

C. PE molecular species profile of E. coli strain SM2-1 complemented with EcPlsC grown in the presence of exogenous oleate.

D. PE molecular species profile of E. coli strain SM2-1 complemented with AfPlsC grown in the presence of exogenous oleate.